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Disruption pressures

One Shot Machine (OS)

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The ideal tool for R&D : the One Shot Cell Disruptor

The OneShot cell disruptor is the ideal tool for R&D.

At approximately 500mm² the OS sits very comfortable on the benchtop but is best suited when used on its tailor made trolley. The OS trolley ensures that the equipment is situated at its optimum working height and is mounted on full swivel and lockable casters that enables safe and free movement of the equipment when needed. The OS is capable of processing volumes from 0.5mL to 8mL per process with a dead loss volume of < 0.1mL per process.

 

The OS is recommended for total volumes of up to 24mL which is ideal for those users who wish to upgrade from small bench-top techniques such as bead beating and sonication. The OS takes advantage of Constant Systems precise and consistent hydraulic control which is fully scalable through the whole product range from 0.5mL single preparation processing to large volume processing at 150L per hour. This precise control ensures that the entire sample is processed at the operators set pressure to ensure accurate and consistent results and in most cases a single process or pass is adequate therefore multiple passes are not required. The OS model can be utilised for processing fluid, re-suspended, viscous, tissue, plant and frozen sample types.

 

Used for processing fast and efficiently small samples of various suspensions : microorganisms, yeasts, filandrous mushrooms, vegetable and mammalian cells but also tissues as leaves, muscles or liver.

  • Quick and easy to use (installation : 10 seconds ; disruption : less than 1 second)

  • Disruption pressure easily and accurately set

  • Pressure consistent and stable during disruption cycle

  • Easily dismantled for manual cleaning and autoclaving

  • Incorporates a disruption head and hydraulic operating and control system

  • Materials in contact with sample : 316 stainless steel or equivalent, synthetic sapphire, EPDM, PTFE, GLFPTFE and PEEk 450G

Important !

The One Shot Head Adaptor

Incorporates the unique capability of One Shot model into the continuous flowing range of Constant Systems cell disrupters.

  • For processing sample in suspension or small "solids"

  • Scale-up and reproducible results

  • Materials in contact with sample are 316L stainless steel

  • Sample volume : from 2 to 10ml

  • Cycle per shot : from 1 to 30 seconds, depending on cell disrupter model

  • Disruption time : from 0,1 to 1 second

  • Pressure : up to 2700 bars

  • Machine weight : 10 kgs

Safety system : disabled if not correctly assembled, no aerosols

Maintenance : the high pressure gasket (or seal) and jet assembly is very specific. In case of damage, the equipment must be sent back to factory for standard replacement.

The One Shot Machine can be equiped with a new anti-Foam cup especially designed to stop definitely all the problems of Foam during the breakage
Ask for more informations.

OS Disruptor
CF

CF1 & 2 Disrupter
for continuous process

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Constant Systems cell disintegrators of the "CF" series are used to continuously break down microorganism and yeast suspensions.


The CF Cell Disruptor offers Continuous Flow Processing with two models, the CF1 and CF2.

Both models offer the same process and differ only by process speed, the CF1 offers up to 6L per hour and the CF2 offers up to 24L per hour processing rates.

Both models benefit from a HMI control, a maximum process pressure of 40kpsi (2700 bar), integrated sample cooling jacket, 200mL inlet reservoir (hopper), auto shut down feature for when the process is complete and both models take advantage of Constant Systems precise and consistent hydraulic control which is fully scaleable through the whole product range from 0.5mL single preparation processing to large volume processing at 150L per hour.

This precise control ensures that the entire sample is processed at the operators set pressure to ensure accurate and consistent results and in most cases a single process or pass is adequate meaning multiple passes are not required. Both models are capable of processing fluid or re-suspended sample types and are utilised for many sample types such as bacteria, yeast and algae.

 

CF1 Model

At approximately 700mm² the CF1 is small enough to bench mount if required but is best suited when used on its tailor made trolley. The CF1 trolley ensures that the equipment is situated at its optimum working height and is mounted on full swivel and lockable casters that enables safe and free movement of the equipment when needed. The CF1 is recommended for process volumes in the range of 15mL through to 10L. For those processing larger volumes in this range then the CF1 can be offered with an integrated peristaltic pump fully controlled through the HMI which will ensure that the inlet reservoir is continually fed whilst recirculating the sample to help avoid any settling until the entire sample is processed.

 

CF2 Model

With a footprint of approx. 700mm² the CF2 is a floor standing model that is recommended for process volumes from 15mL through to 100L. The CF2 is offered with an integrated peristaltic pump as standard. The CF2 is mounted on full swivel and lockable castors that enables safe and free movement of the equipment when needed.

 

 

HFR  for production scale

Ideal Disruptor in Production phase with flow rates up to 140L / h

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The HFR is the ideal production model and offers the same lysis efficiency as the standard CSL systems across the full range of processing pressures yet can provide a vastly superior flow rate.

With lysis efficiencies of up to 95% or more and flow rates of up to 150 L/hr, this system can make short work of processing the entire contents of a production scale fermenter or bioreactor, even without prior pelleting and re-suspending.

The intuitive user interface offers complete control of the flow rate while the flow path accumulator ensures that the entire sample is processed smoothly and efficiently. This machine has been shown to give the same lysis results as the CF Range when processing tough to break cells types such as S. cerevisiae and N. oculata and the innovative heat exchange system ensures that the processed sample is completely chilled upon recovery when fitted with a circulating chiller.

  • Incorporates our unique disruption technique as well as our very precise Hydraulic Operation Control system

  • Easy and precise disruption pressure adjustment with a range of up to 40 Kpsi as standard

  • Digital display

  • Automatic stop at the end of the process

  • Different flow rates available depending on the maximum pressure desired

  • Constant and stable pressure during the grinding cycle

  • Standard tri-clamp connections for input / output - allow direct connections to peripheral equipment installed upstream and downstream

  • Fully scalable on the whole range of Constant Systems Ltd  disrupters

  • Materials in contact with the sample : Duplex stainless steel 316L and F51 or equivalent, synthetic sapphire, EPDM, GLFPTFE & PEEK 450G *

  • IQ / OQ available on request

HFR

Videos

Cell Disrupters Videos
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